43 湖南省地方标准 羊肚菌制种技术规程 Technical regulations for the production of morchella strainsICS CCS 65.020 B 30 DB43/T2248—2021 发 布 湖南省市场监督管理局2021-12发布-29 2022-03实施-29 DB43/T 2248—2021 I 目 次 前言 ································ ································ ································ ························ Ⅲ 1 范围 ································ ································ ································ ····················· 1 2 规范性引用文件 ································ ································ ································ ······ 1 3 术语与定义 ································ ································ ································ ············ 1 4 环境卫生 ································ ································ ································ ··············· 1 5 菌种选择 ································ ································ ································ ··············· 1 6 母种生产 ································ ································ ································ ··············· 1 7 原种生产 ································ ································ ································ ··············· 2 8 栽培种生产 ································ ································ ································ ············ 3 9 质量检验 ································ ································ ································ ··············· 3 10 废弃物处理 ································ ································ ································ ··········· 4 11 档案记录 ································ ································ ································ ·············· 4 附录A（资料性） 常用母种培养基 配方 ································ ································ ·········· 5 附录B（资料性） 常用原种培养料配方 ································ ································ ·········· 6 附录C（资料性） 常用栽培种培养料配方 ································ ································ ······· 7 附录D（资料性） 羊肚菌制种生产记录表 ································ ································ ······· 8 附录E（资料性） 羊肚菌制种栽培农艺性状检 测项目 ································ ························ 9 DB43/T 2248—2021 II DB43/T 2248—2021 III 前 言 本文件按照 GB/T 1.1 —2020《标准化工作导则 第1部分：标准化文件的结构和起草规则》的规 定起草。 请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。 本文件由湖南省农业农村厅提出。 本文件由湖南省农业标准化技术委员会归口。 本文件起草单位： 湖南金芙农业科技有限公司、 衡阳桃源特色种养专业合作社、 衡阳市农业农村局、 衡阳市蔬菜研究所。 本文件主要起草人：胡国平、龙新、孔英戈、杨彩霞、郑四清、李勤、郑婷、周强、龙耀、乔慧、 刘香、吕秀娟、徐三阳。 DB43/T 2248—2021 IV DB43/T 2248—2021 1 羊肚菌制种技术规程 1 范围 本文件规定了羊肚菌制种的环境卫生、菌种选择、母种生产、原种生产、栽培种生产、质量检验、 废弃物处理与档案记录的要求。 本文件适用于湖南省羊肚菌制种生产。 2 规范性引用文件 下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。 其中， 注日期的引用文件， 仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本 文件。 GB 5749 生活饮用水卫生标准 GB/T 12728 食用菌术语 NY/T 528 食用菌菌种生产技术 规程 NY/T 1742 食用菌菌种通用技术要求 NY/T 1846 食用菌菌种检验规程 NY/T 1935 食用菌栽培基质质量安全要求 3 术语与定义 本文件没有需要界定的术语和定义。 4 环境卫生 环境卫生应符合 NY/T 528 的规定。 5 菌种选择 菌种应从具有相应资质的供种单位购买，菌种质量应符合 NY/T 1742 的要求；自制菌种应符合《食 用菌菌种管理办法》的规定。 6 母种生产 6.1 工艺流程 培养基配制→分装→灭菌→摆斜面冷却→接种→培养→成品。 DB43/T 2248—2021 2 6.2 培养基配制 常用母种培养基及其配方参见附录 A。 6.3 分装与灭菌 采用规格为 18 mm×180 mm或20 mm×200 mm的玻璃试管，试管用硅胶塞封口。将配制好的培养基 立即分装，装量为试管长度的 1/4～1/5。试管口应保持干净，随后塞入硅胶塞，并用牛皮纸包扎。将 分装包扎好的试管直立放入高压灭菌锅内（压力 0.12 Mpa～0.15 Mpa、温度121 ℃～126 ℃） ，灭菌 25 min～30 min。 6.4 斜面摆放 灭菌结束后自然冷却，压力表指针降至 O Mpa时，打开排气阀，在培养基温度降到 55 ℃～65 ℃ 时取出试管摆斜面，斜面长度不超过试管长度的 2/3。 6.5 接种 接种设施设备消毒流程应符合 NY/T 528 的规定。接种时应无菌操作，接种后及时贴好标签，标签 应注明菌种名称及接种时间。 6.6 培养 接种后置于培养箱或培养室培养，控制温度 18 ℃～22 ℃，避光培养 5 d～7 d，定期检查，清除 污染试管，待菌丝长满试管斜面，稳定 3 d～5 d即可用于原种生产。 7 原种生产 7.1 工艺流程 培养料配制→装瓶→灭菌→冷却→接种→培养→成品。 7.2 培养料选择 7.2.1 主料 木屑、小麦、腐殖土等培养料质 量应符合 NY/T 1935 的规定。 木屑为阔叶树硬杂木粗木屑，颗粒在 0.5 cm×0.8 cm左右，提前堆放发酵腐熟；腐殖土宜为植物 枝叶在土壤中经过微生物分解发酵后形成的营养土或草炭土； 小麦应新鲜无霉变、 无虫害， 颗粒度饱满。 7.2.2 辅料 麸皮、石膏、石灰、玉米芯等辅料质量及贮藏应符合 NY/T 1935 的规定。 7.3 生产用水 应符合GB 5749要求。 7.4 培养料的配制 小麦先用 1％的石灰水煮 30 min左右至麦子煮胀，