ICS 11.220 CCS B 41 云 53 南 省 地 方 标 准 DB53/T 1059.1—2021 滇金丝猴检疫技术 第 1 部分：产气荚膜 梭菌实验室检测技术规范 Quarantine technique for Rhinopithecus bieti —Part 1: Technical specifications for laboratory examination of Clostridium perfringens 2021 - 09 - 30 发布 云南省市场监督管理局 2021 - 10 - 14 实施 发 布 DB53/T 1059.1—2021 目  次 前言..................................................................................................................................................................... III 引言..................................................................................................................................................................... Ⅴ 1 范围................................................................................................................................................................. 1 2 规范性引用文件.............................................................................................................................................1 3 术语和定义.....................................................................................................................................................1 4 缩略语............................................................................................................................................................. 2 5 总则................................................................................................................................................................. 2 6 产气荚膜梭菌分型.........................................................................................................................................3 7 仪器设备和试剂.............................................................................................................................................3 7.1 7.2 7.3 8 采样用品..................................................................................................................................................3 实验材料..................................................................................................................................................3 实验设备..................................................................................................................................................3 样品................................................................................................................................................................. 3 8.1 8.2 8.3 9 采样要求..................................................................................................................................................4 采样程序..................................................................................................................................................4 样品保存与运输......................................................................................................................................4 试验步骤......................................................................................................................................................... 4 9.1 9.2 9.3 9.4 9.5 10 细菌分离培养及培养特性观察..............................................................................................................4 革兰染色和芽孢染色镜检......................................................................................................................4 细菌生化试验..........................................................................................................................................4 小鼠接种试验..........................................................................................................................................4 16S rRNA 基因与 4 种毒素基因的 PCR 扩增及测序分析...............................................................5 试验数据处理...............................................................................................................................................5 10.1 10.2 10.3 10.4 10.5 10.6 细菌培养结果判定................................................................................................................................5 染色镜检结果判读................................................................................................................................5 生化试验结果判定................................................................................................................................6 小鼠毒性试验结果判定........................................................................................................................6 毒素中和试验结果判定.............................................