ICS65.020.30 CCSB41DB2305 双鸭山市地方标准 DB2305/T020—2024 __________________________________________________________________________________________ 牛病毒性腹泻病毒PCR检测规程 2024-08-20发布 2024-09-20实施__________________________________________________________________________________________ 双鸭山市市场监督管理局发布 DB2305/T020—2024 目次 前言·····························································································································Ⅱ 1范围································································································································1 2规范性引用文件·················································································································1 3术语和定义·······················································································································1 4缩略语·····························································································································1 5主要仪器设备····················································································································1 6试剂································································································································2 7样品的采集与处理··············································································································3 7.1样品采集·······················································································································3 7.2样品处理·······················································································································3 8样品病毒核酸RNA提取········································································································3 9PCR扩增···························································································································3 9.1引物·····························································································································3 9.2反应条件·······················································································································4 10结果判定························································································································4 10.1检测结果成立条件·········································································································4 10.2结果判定·····················································································································4 11实验室无害化处理············································································································4 11.1病料的无害化处理·········································································································4 11.2实验室废弃物处理·········································································································4 11.3实验室分区··················································································································4 11.4实验室生物安全管理······································································································4 DB2305/T020—2024 III前言 本文件按照GB/T1.1-2020《标准化工作导则第1部分:标准化文件的结构和起草规则》的规定起草。 请注意本文件的某些内容可能涉及专利。本文的发布机构不承担识别专利的责任。 本文件由双鸭市农业农村局提出并归口。 本文件起草单位：双鸭市农业技术综合服务中心、双鸭山市基本建设项目服务中心、黑龙江农业职 业技术学院。 本文件主要起草人：王宇婷、白鹭、刘云、张红军、杜航、张荣翔、秦藕菊。